The aim of this workshop is to show, through lectures and practical courses, the Solutions for 3D reconstruction in Cryo-Electron Microscopy. The lectures will cover single particle analysis, cryo-tomography of isolated specimens and cryo-tomography of structures inside a vitreous cryo-section or lamella and cryo-CLEM. The practical courses will show to the participants how to do sample preparation (high pressure freezing, plunge freezing, vitreous sectioning and cryo-fibbing), image acquisition in cryo-EM single-particle and cryo-tomography and 3D-reconstruction.